Fig. 1

Chronic social defeat stress (CSDS) induces depressive behavior in mice. A Schematic diagram of the experimental design representing all four groups of mice. While, in C-H two separate group namely (susceptible and resilient mice) generated according to their performance in the behavior tests with respect to the naive control group. C56BL/6 mice were subjected to CSDS-induced depression and assessed by the social interaction test (SIT), swimming test (FST) and sucrose preference test (SPT). B The figure represents the percent change in the weight of each mouse group recorded every 5 days. (C-H) Behavior tests contain three groups (control, depressed and resilient). C Representative movement map of SIT information for control, susceptible and resilient mice generated with PANLAB software. D-E Statistical analysis of movement parameters in the SIT by calculating the time spent by each mouse in the interaction or corner zones after undergoing the CSDS procedure. F Social interaction (SI) ratio % was calculated by using the formula described in the methods section to differentiate each mouse into depressed or resilient by analyzing the total time spent in each zone. G The FST results were analyzed by observing the immobility time of each mouse from the total recorded swimming duration of 4 min. H The sucrose percent ratio was calculated according to the formula added in the material and method by performing the SPT. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001 (two-way ANOVA). Data are representative of three independent experiments with three biological replicates (mean ± SEM)