Fig. 2

Evaluating BCG efficacy by organ pathology and Mycobacterium growth inhibition assay (MGIA) assay (A, B) Spleen and lung organ index calculated by taking the ratio of the organ weight to total weight of each mouse. Illustrated pictures from each group showing lung and spleen taken for weight and organ physiological studies. C H&E staining of lung sections, 200x magnification (scale bar: 200 μm). D, E CFU in the lung and spleen of the BCG infected groups. F Pictorial diagram to represent the MGIA procedure as discussed in the methods section. G The visualization of effector T cells in action against intracellular RFP-H37Ra in BMDM cells using fluorescence microscopy in all four groups of mice, 100x magnification (Scale bar:100 μm). H Viable intracellular RFP-H37Ra inside BMDMs with and without coculture of effector T cells was evaluated by counting CFUs in all four groups of mice. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001. Statistical analysis in A and B was calculated by one-way ANOVA and H by two-way ANOVA, while D and E were calculated by unpaired t test. Data are representative of three independent experiments with three biological replicates (mean ± SEM)