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Fig. 1 | Animal Diseases

Fig. 1

From: Identification of novel serological agents for porcine deltacoronavirus infection based on the immunogenic accessory protein NS6

Fig. 1

Expression and purification of PDCoV NS6 and S1 proteins. a SDS‒PAGE analysis of purified recombinant PDCoV NS6 protein. M: protein marker, empty vector pCold I (lane 1), unpurified NS6 protein (lane 2), purified NS6 protein (lane 3). b WB analysis of empty vector pCold I (lane 1) and purified NS6 protein (lane 2) using anti-His monoclonal antibody (mAb) and mouse anti-NS6 pAb, respectively; M: marker. c WB analysis of recombinant S1 protein. An 85-kDa band was detected by an anti-His mAb or a rabbit anti-S1 pAb; M: protein marker. Red arrows indicate the target protein bands

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