Fig. 4

Validation of the NS6-iELISA by western blot. a Identification of PDCoV-negative and positive sera by western blot (WB). Bands appeared at approximately 13 kDa in positive serum samples and no target bands in negative samples. M: protein marker. Red arrows indicate the target protein bands. b, c ROC curve using the WB as the diagnostic standard. Serum samples were classified as negative or positive based on the results of WB. Data presented as ELISA OD values ± SD