Isolation of four carbapenem-resistant gram-negative species from a single fly

Animal Diseases

Table 1 Minimum inhibitory concentration (µg/mL) profiles of isolates in this study

Isolates^a	Species	IPM	MEM	CMZ	CAZ	CTX	TZP	SCF	CAV	FEP	PB	TGC	CIP	AK	ATM
36-2-1	Myroides odoratimimus	64	8	128	64	64	32/4	> 256/128	64/4	16	> 8	0.5	≤ 1	> 128	> 128
36-2-2	Proteus mirabilis	> 128	128	64	> 128	> 128	32/4	256/128	> 64/4	> 64	> 8	4	≤ 1	> 128	> 128
36-1-1	Escherichia coli	8	16	> 128	> 128	> 128	> 256/4	> 256/128	> 64/4	64	1	1	≤ 1	≤ 4	≤ 4
36-1-2	Providencia manganoxydans	128	64	16	> 128	> 128	> 256/4	> 256/128	> 64/4	> 64	> 8	≤ 0.25	≤ 1	≤ 4	≤ 4
EC600	Escherichia coli	≤ 1	≤ 1	≤ 2	≤ 2	≤ 4	≤ 8/4	≤ 8/4	≤ 0.5/4	≤ 4	≤ 0.5	≤ 0.25	≤ 1	≤ 4	≤ 4
36-1-1-TC	Escherichia coli	8	16	8	> 128	> 128	128/4	256/128	> 64/4	32	≤ 0.5	≤ 0.25	2	≤ 4	≤ 4

Abbreviations: IPM Imipenem, MEM Meropenem, CMZ Cefmetazole, CAZ ceftazidime, CTX Cefotaxime, TZP Piperacillin/tazobactam, SCF Sulbactam/cefoperazone, CAV Ceftazidime/avibactam, FEP Cefepime, PB Polymyxin B, TGC Tigecycline, CIP Ciprofloxacin, AK Amikacin, ATM Aztreonam
^aIsolates 36-2-1, 36-2-2, 36-1-1 and 36-1-2 were isolated from a single fly in this study; E. coli EC600 was the recipient used in the conjugation assay; E. coli 36-1-1-TC was the transconjugant that acquired the bla_NDM-5 gene from the donor isolate E. coli 36-1-1