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Fig. 2 | Animal Diseases

Fig. 2

From: The QseB/QseC two-component system contributes to virulence of Actinobacillus pleuropneumoniae by downregulating apf gene cluster transcription

Fig. 2

Analysis of QseB protein. Lane 1, cell lysate of BL21 (DE3) containing plasmid pET-30a; Lane 2, lysate of BL21 (DE3) containing plasmid pET-qseB without isopropyl-β-D-thiogalactoside (IPTG) induction; Lane 3, cell lysate of BL21 (DE3) containing plasmid pET-qseB with IPTG induction at 25 °C for 4 h; Lane 4 and 5, supernatant (lane 4) and inclusion body (lane 5) of cell lysate of BL21 (DE3) containing plasmid pET-qseB with IPTG induction at 25 °C for 4 h; Lane 6, purified QseB protein

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