Fig. 4

The TAB3 mediates the indirect interaction between OASL and Rv0127. A The interaction network between M. tuberculosis protein and host proteins constructed by Y2H; B Validation of the interaction between TAB3 and Rv0127 by Y2H. Co-transformation of pmBD empty vector with pmAD vector containing T and co-transformation of the pmAD empty vector with pmBD vector containing p53 were used as negative controls. Co-transformation of pmAD vector containing T and pmBD vector containing p53 was used as positive controls. C–D Validation of protein-protein interactions by co-IP experiments. HEK 293 T cells were co-transfected with V5-tagged TAB3 and Flag-tagged Rv0127, OASL2. Cells were harvested for Co-IP assays 36 h after transfection. Co-transfection of V5-tagged TAB3 with Flag-tagged empty vector and Flag-tagged Rv0127 with V5-tagged empty vector, Flag-tagged OASL2 with V5-tagged empty vector were used as negative controls; E The expression of TAB3 in different THP-1 cells before and after M. tuberculosis infection; F Schematic of how OASL may promote M. tuberculosis survival in macrophages. All experiments were performed in triplicate. The values of *p < 0.05 were considered a statistically significant difference